Since the early days of radioisotope sequencing, a wide variety of new DNA sequencing techniques have emerged. New methods are aimed at reducing the amount of sample needed, improving the accuracy, and reducing the amount of time needed to generate a sequence. This text discusses the different analytical DNA sequencing techniques, as well as exciting applications of DNA sequencing. Advances in DNA separations using advanced capillary electrophoresis and microchip sequencing are presented. Chapters cover improvements in the detection of DNA sequencing fragments, including types of fluorescence detection, the use of mass spectrometry, and single molecule detection. The uses of DNA sequencing in forensics, the sequencing of ancient DNA, and genome sequencing are also included.