This two-volume set LNCS 13344 and 13345 constitutes the proceedings of the 13th International Conference on Advances in Swarm Intelligence, ICSI 2022, which took place in Xi'an, China, in July 2022. The theme of this year's conference was "Serving Life with Swarm Intelligence".The 85 full papers presented were carefully reviewed and selected from 171 submissions. The papers of the first part cover topics such as: Swarm Intelligence and Nature-Inspired Computing; Swarm-based Computing Algorithms for Optimization; Particle Swarm Optimization; Ant Colony Optimization; Differential Evolution; Genetic Algorithm and Evolutionary Computation; Fireworks Algorithms; Brain Storm Optimization Algorithm; Bacterial Foraging Optimization Algorithm; DNA Computing Methods; Multi-Objective Optimization; Swarm Robotics and Multi-Agent System; UAV Cooperation and Control; Machine Learning; Data Mining; and Other Applications.