Chemical Biology is devoted to understanding the way that Biology works at the molecular level. This is a problem-driven multi-discipline, incorporating as it does Organic, Physical, Inorganic, and Analytical Chemistry alongside never emerging molecular disciplines. In recent years, Chemical Biology has emerged as a vibrant and growing multi-discipline distinct from Biochemistry that is focused on the quantitative analyses of the structure and functions of biological macromolecules and macromolecules lipid assemblies, at first in isolation, then in vitro and in vivo. The second edition of the Essentials of Chemical Biology begins with a thorough introduction to the structure of biological macromolecules and macromolecular lipid assemblies, before moving on to the principles of chemical and biological synthesis, followed by descriptions of a comprehensive variety of research techniques and experimental methods. In addition, the second edition now includes new sections on the behaviour of biological macromolecules and macromolecular lipid assemblies in cells in vitro and in organisms in vivo. Given this, the second edition of the Essentials of Chemical Biology promises to cement itself as the leading introduction to Chemical Biology, incorporating descriptions of cutting-edge research wherever appropriate. Hence, readers of the second edition of the Essentials of Chemical Biology will find: a general expansion in understanding of basic molecular mechanisms in Biology moving towards cellular and organismal mechanisms, entirely new chapters covering miniaturization and array technologies. Chemical Cell Biology, and the interface between Chemical Biology and Nanotechnology, updates to chapters reflecting recent research developments, an increased engagement with medical applications Essentials of Chemical Biology is ideal for advanced undergraduates or (post) graduate students in Chemical Biology and adjacent fields.