This book provides an overview of single-cell
isolation, separation, injection,
lysis and dynamics analysis as well as a study of their heterogeneity using
different miniaturized devices. As an important part of single-cell analysis, different
techniques including electroporation,
microinjection, optical trapping, optoporation, rapid electrokinetic patterning
and optoelectronic
tweezers are described in detail. It presents different fluidic
systems (e.g. continuous micro/nano-fluidic devices, microfluidic cytometry)
and their integration with sensor technology, optical and hydrodynamic stretchers etc.,
and demonstrates the applications of single-cell analysis in systems biology,
proteomics, genomics, epigenomics, cancer transcriptomics, metabolomics,
biomedicine and drug delivery systems. It also discusses the future challenges
for single-cell analysis, including the advantages and limitations.



This book is enjoyable reading material while
at the same time providing essential information to scientists in academia and
professionals in industry working on different aspects of single-cell
analysis.



Dr. Fan-Gang Tseng is a Distinguished Professor of
Engineering and System Science at
the National Tsing Hua University, Taiwan.



Dr. Tuhin Subhra Santra is a Research Associate at the California Nano Systems Institute, University of California at Los Angeles, USA.