This book is primarily aimed at newcomers to the field of developmental biology, but is also useful to experienced workers. In this volume, the South African clawed frog Xenopus Iaevis and the zebrafish Brachydanio rerio serve as the model organisms for the developmental studies. Both species are well suited to investigations of early vertebrate development because of their embryos develop rapidly and externally, but each also has its set of disadvantages. This methods manual includes experimental approaches which can be applied to both species, since many laboratories also use these two models for similar studies. For example, the approaches for the analysis of steady-state mRNA levels by RT-PCR or PNase protection are identical for Xenopus and zebrafish, but for whole-mount in situ hybridization, two tissue explant and transplant techniques, quantitative and spatial analysis of mRNA and immunohistochemistry. The final chapters focus on more specialized methods for expression and promoter analysis, such as the analysis of transcription control in early development.
The methods and techniques presented in this volume will provide a sound basis for the majority of studies in this field.
- ISBN:
- 9780896037908
- 9780896037908
-
Category:
- Zoology: Vertebrates
- Format:
- Hardback
- Publication Date:
-
03-08-1999
- Language:
- English
- Publisher:
- Humana Press Inc.
- Country of origin:
- United States
- Pages:
- 217
- Dimensions (mm):
- 235x155x16mm
- Weight:
- 0.56kg
This title is in stock with our Australian supplier and should arrive at our Sydney warehouse within 2 - 3 weeks of you placing an order.
Once received into our warehouse we will despatch it to you with a Shipping Notification which includes online tracking.
Please check the estimated delivery times below for your region, for after your order is despatched from our warehouse:
ACT Metro: 2 working days
NSW Metro: 2 working days
NSW Rural: 2-3 working days
NSW Remote: 2-5 working days
NT Metro: 3-6 working days
NT Remote: 4-10 working days
QLD Metro: 2-4 working days
QLD Rural: 2-5 working days
QLD Remote: 2-7 working days
SA Metro: 2-5 working days
SA Rural: 3-6 working days
SA Remote: 3-7 working days
TAS Metro: 3-6 working days
TAS Rural: 3-6 working days
VIC Metro: 2-3 working days
VIC Rural: 2-4 working days
VIC Remote: 2-5 working days
WA Metro: 3-6 working days
WA Rural: 4-8 working days
WA Remote: 4-12 working days
Share This Book: