Part I: Detection and Quantification of pADPr

1. Fluorescence-Based Analyses of Poly(ADP-Ribose) Length by Gel Electrophoresis, High Performance Liquid Chromatography, and Capillary Electrophoresis

Mohsen Badiee, Audrey Boutonnet, Dat Phan, and Anthony K.L. Leung

2. Detecting and Quantifying pADPr In Vivo

Andrew M. Lamade, Yaming Chen, Carla J. Johnson, Hülya Bayır, and Robert S.B. Clark

3. Quantitative Analysis of Nuclear Poly(ADP-Ribose) Dynamics in Response to Laser-Induced DNA Damage

Christopher A. Koczor, Kate M. Saville, Rasha Q. Al-Rahahleh, Joel F. Andrews, Jianfeng Li, and Robert W. Sobol

4. Analyzing PARP1 Activity: Small Molecule Reactants and Attached Chains of Poly(ADP-Ribose)

Johannes Rudolph and Karolin Luger

5. Detecting Poly(ADP-Ribose) In Vitro and in Cells Using PAR-Trackers

Sridevi Challa, Amy L. Whitaker, and W. Lee Kraus

6. An Enzyme-Linked Immunosorbent Assay to Quantify Poly(ADP-Ribose) Level In Vivo

Chieri Ida, Sachiko Yamashita, Takayuki Eguchi, Yasuhito Kuroda, Setsu Nakae, Yoshisuke Nishi, Kazuo Kamemura, Tsuyoshi Shirai, Tamio Mizukami, Masakazu Tanaka, Joel Moss, and Masanao Miwa

7. Subcellular Quantitation of ADP-Ribosylation by High Content Microscopy

Lukas Muskalla, Anka Güldenpfennig, and Michael O. Hottiger

8. A Simple Method to Study ADP-Ribosylation Reversal: From Function to Drug Discovery

Johannes Gregor Matthias Rack and Ivan Ahel

Part II: Identification of Protein Targets

9. Immunoprecipitation Using Mono-ADP-Ribosylation Specific Antibodies

Helen Dauben and Ivan Matic

10. A Clickable NAD⁺ Analog-Based Assay of Poly(ADP-Ribosyl)ated Proteins

Dongsheng Yao, Heba Ahmed, and Junqi Song

11. Functional Analysis of Histone ADP-Ribosylation In Vitro and in Cells

Dan Huang, Andrea D. Edwards, Xuan Gong, and W. Lee Kraus

Part III: Functional Analysis

12. Studying the Immunomodulatory Functions of PARP1 and PARP2 in Mouse Models of Cancer

Nura Lutfi, Carlos Martínez, and José Yélamos

13. Methods for Investigating Transient Receptor Potential Melastatin-2 (TRPM2), a Cation Channel Activated by ADP-Ribose and Involved in Cell Death

Hannah K. Hall and David W. Koh

14. Methods to Assess the Role of PARPs in Regulating Mitochondrial Oxidative Function

Tünde Kovács, Boglárka Rauch, Edit Mikó, and Péter Bai

15. Characterizing ADP-Ribosylation Sites Using Af1521 Enrichment Coupled to ETD-Based Mass Spectrome