Techniques for Downstream process for Biologic Drugs and Vaccines represents comprehensive technologies involved in processing postharvest broth to separate the target biological therapeutic product of extracellular or intercellular in nature, in its highest purification form to make its acceptability by end users with utmost confidence, resulting in the relief of life-threatening communicable diseases and non-communicable diseases. Conceptual development of biotechnology has taken a new shape and style with the integration of medical sciences, physical science, and engineering. This integration has allowed for the development of microbial or cell line process technology and application for large-scale isolation and purification of metabolites or vaccines through the fermentation process. These metabolites are in the form of biologic drugs which are widely used in biopharmaceutical, biochemical, food, nutraceuticals, and diagnostics. The current global market for vaccines, especially the vaccine for COVID-19 is tremendous. Bivalent oral polio vaccine, diphtheria, tetanus-containing, and measles-containing vaccines have a high demand internationally. In this connection, recombinant DNA technology and protein engineering are highly helpful in the production of quality bio-products.The technologies involved in the post-harvesting of fermented broth are explained in a simplified manner with different types of case studies to make the non-engineering students and scientists easily capture the basic principle of biomass processing technologies and their application on a new project related to therapeutic bio-products development and manufacturing.