This book collects papers presented in the Invited Workshop, “Liutex and Third Generation of Vortex Definition and Identification for Turbulence,” from CHAOS2020, June 9-12, 2020, which was held online as a virtual conference. Liutex is a new physical quantity introduced by Prof. Chaoqun Liu of the University of Texas at Arlington. It is a vector and could give a unique and accurate mathematical definition for fluid rotation or vortex. The papers in this volume include some Liutex theories and many applications in hydrodynamics, aerodynamics and thermal dynamics including turbine machinery.As vortex exists everywhere in the universe, a mathematical definition of vortex or Liutex will play a critical role in scientific research. There is almost no place without vortex in fluid dynamics. As a projection, the Liutex theory will play an important role on the investigations of the vortex dynamics in hydrodynamics, aerodynamics, thermodynamics, oceanography, meteorology, metallurgy, civil engineering, astronomy, biology, etc. and to the researches of the generation, sustenance, modelling and controlling of turbulence.