Peptides and proteins are crucial biomolecules in life. The manifold functions they carry out range from molecular recognition and signaling to catalysis and immune response. However, the native systems are limited to a reduced toolbox of chemical functionalities as well as tridimensional structures. Widening these toolboxes could pave the way to engineer peptides and proteins with enhanced properties compared to their native counterparts and/or with structures and functions unprecedented in Nature. Advances in the chemical and biological synthesis of peptides and proteins, in computational tools, in molecular biology and in high-throughput screening methods are making this realm possible.

This book aims to give an overview of the last developments in the field of peptide and protein engineering. It comprises a collection of chapters that span from the production of simple non-proteinogenic building blocks and peptidic scaffolds of different sizes and structures to more complex systems including peptide-based nanomaterials, enzymes and artificial metalloenzymes. Different strategies are described where chemical and biological tools have been developed and combined to attain the desired properties and sought functionalities.

The diverse systems described in this book highlight the progress in this important field and represent the starting points for the development of functional biomolecules, biomaterials and hybrid systems capable of addressing key societal challenges of our times in relevant areas such health, environment and energy.

Contents:

• Cis-Amide Stabilizing Proline Analogs: Synthesis and Applications (Isita Banerjee, Indranil Duttagupta, Abhishek Gupta, and Surajit Sinha)


• Antimicrobial Peptides and Peptidomimetics as Treatment Option for Helicobacter pylori Infection (Hamza Olleik, Josette Perrier, Akram Hijazi, Elias Baydoun, and Marc Maresca)


• Antimicrobial Ribosomally Synthesized and Post-Translationally Modified Peptides as a Source of Alternatives to Antibiotics: A Focus on the Sactipeptides and Ranthipeptides Subclasses (Clarisse Roblin, Pierre Rousselot-Pailley, Victor Duarte, Josette Perrier, and Mickael Lafond)


• Peptide Nanotubes as Dynamic Materials (Federica Novelli and Juan R Granja)


• Peptide and Protein-Based Nanomaterials for Theranostics (Pascal Tomasella, Lorena Maria Cucci, Vanessa Sanfilippo, and Cristina Satriano)


• Engineering Peptidyl and Protein Glutathione Peroxidase Mimics (Nicolas Delsuc)


• DNA Recognition with Artificial Metallopeptides (Jacobo Gómez-González, Ana Alcalde, Miguel Vázquez López, and M Eugenio Vázquez)


• Recombinant Production, Isotopic Labeling, Purification and NMR Characterization of Cysteine-rich Domains: The Case of the Death Receptors Involved in Cancer Treatment (Antoine Baudin, Anne Guichard, Lua Sobron, Gavin W Collie, Sabrina Rousseau, Gilles Guichard, and Benoît Odaert)


• Substrate Binding Free Energy Profiles: Probing Enzyme-Substrate Interactions by Molecular Dynamics Simulations (Juergen Pleiss and Henrique Carvalho)


• Peptide-based Artificial Metalloenzymes by Design (Ornella Maglio, Marco Chino, Daniele D'Alonzo, Linda Leone, Flavia Nastri, and Angela Lombardi)


• Biohybrid Catalysts Harboring Synthetic Metal Complexes within β-Barrel Proteins (Akira Onoda, Shunsuke Kato, and Takashi Hayashi)


• Chemistry for a Better Life: Elaborating Artificial Metalloenzymes for Eco-compatible Chemical Synthesis Procedures and Therapeutic Applications (Frédéric Avenier, Wadih Ghattas, Rémy Ricoux, and Jean-Pierre Mahy)

Readership: Students and researchers in peptide and protein engineering, peptide-based nanotubes and nanomaterials, proteins, denovo peptide and protein design, artificial metalloenzymes and molecular dynamics simulations.

Key Features:

• Overview of the last developments in the field of peptide and protein engineering


• Combination of biological and chemical tools


• Contributions by experts of the field