The third edition of this popular problem-solving guide for this widely-used method includes eleven completely new examples and

several updated ones, adding up to 100 contributions about pitfalls and errors in HPLC. Each example is presented on a double page with the

text on the left-hand and a fi gure on the right-hand side, true to the motto 'a picture says more than a thousand words'. In addition, the

author presents essential fundamentals as well as helpful strategies, such as equipment tests or quality assurance processes.

New in this edition

* Variability of the standard deviation

* Infl uence of the acid type and concentration in the eluent

* Water as an unintentional additive in the mobile phase

* Inadequate purity of mobile phase water

* Incomplete degassing

* Inadequate stabilization of the extraction solvent

* Tailing of phosphate compounds in the presence of steel

* Different detection properties of diastereomers

* Detector overload in ELSD

* System suitability test

* From repeatability to reproducibility

A must-have resource for all users - showing how to use HPLC efficiently and obtain reliable results.