FAO has developed the "Practical guide for the application of the Genebank Standards for Plant Genetic Resources for Food and Agriculture: Conservation of orthodox seeds in seed genebanks" to be used as a companion volume to the Genebank Standards for Plant Genetic Resources for Food and Agriculture. The action steps of the genebank workflow are presented in a sequential manner and provide guidance on the complex steps and decisions required when operating a seed genebank. The accompanying summary charts for the respective action steps underscore the intended use of this practical guide as a handbook for routine genebank operations for the conservation of orthodox seeds. While this practical guide is particularly useful for genebank technicians for their day-to-day activities, it may also be used as a basis for the development of standard operating procedures and quality management systems. Genebank managers will also find it useful for conducting training exercises.