This volume LNCS 14240 constitutes the refereed proceedings of the 30th International Symposium on String Processing and Information Retrieval, SPIRE 2023, held in Pisa, Italy, during September 26–28, 2023.

The 31 full papers presented were carefully reviewed and selected from 47 submissions. They cover topics such as: data structures; algorithms; constrained Substring complexity; data compression codes; succinct k-spectra; and LCP array of wheeler DFAs.